Peptide Calculator

Peptide concentration is calculated using the formula:
 
Concentration (mcg/ml) = Amount of peptide (mg) × 1000 / Volume of water (ml)
 
For example, if you have 5 mg of peptide and add 2 ml of water, the concentration will be:
 
5 mg × 1000 / 2 ml = 2500 mcg/ml
 
This means that each ml of solution contains 2500 mcg of peptide, and each 0.1 ml contains 250 mcg of peptide.

For peptide reconstitution, you should use bacteriostatic water for injection. This water contains 0.9% benzyl alcohol which acts as a preservative and prevents bacterial growth, allowing for longer storage of reconstituted peptide (usually 2-4 weeks in the refrigerator).
 
Bacteriostatic water can be purchased at most pharmacies without a prescription. Do not use regular distilled water or water for injection without preservatives, as the reconstituted peptide will be susceptible to bacterial contamination and will have a very short shelf life (24-48 hours).

The shelf life of reconstituted peptide depends on several factors:
 
Type of peptide – Some peptides are more stable than others
Type of solvent – Bacteriostatic water allows for longer storage
Storage conditions – Temperature, light, etc.
 
Generally, peptides reconstituted with bacteriostatic water can be stored in the refrigerator (2-8°C) for up to 5 years.

Before reconstitution (powder form):
Most peptides in powder form can be stored at room temperature for a short time (during transport)
For long-term storage, keep them in the refrigerator (2-8°C)
Some peptides require storage in the freezer (-20°C)
Avoid exposure to light, moisture, and high temperatures
 
After reconstitution (solution):
Always store in the refrigerator (2-8°C)
Protect from light
Do not freeze reconstituted peptides
Use within the recommended timeframe

Peptides are sensitive molecules that can be easily damaged by mechanical forces such as shaking. Shaking can cause:
 
Denaturation – a change in peptide structure that reduces its effectiveness
Aggregation – formation of clusters of peptide molecules
Oxidation – a chemical reaction that can damage the peptide
 
Instead of shaking, gently roll the vial between your palms or rotate it lightly to help dissolve the peptide. This process may take several minutes, but it is crucial for preserving the integrity and effectiveness of the peptide.

Subcutaneous (under the skin) injection:
Injected into fatty tissue under the skin
Uses a shorter needle (usually 26-31G, length 8-13 mm)
Inserted at a 45-degree angle
Slower peptide absorption
Less painful and simpler for self-administration
Suitable for most peptides
 
Intramuscular injection:
Injected directly into muscle
Uses a longer needle (usually 22-25G, length 25-38 mm)
Inserted at a 90-degree angle
Faster peptide absorption
May be more painful and harder for self-administration
Suitable for certain peptides that require faster absorption
 
Most peptides are administered subcutaneously due to the simplicity and safety of application. Always follow the specific instructions for the peptide you are using.

A properly reconstituted peptide should have the following characteristics:
 
Clear solution – without visible particles or cloudiness
Colorless or slightly yellowish – depending on the type of peptide
No sediment – all powder should be completely dissolved
 
If you notice cloudiness, sediment, unusual color, or visible particles after gently rolling the vial for several minutes, it is possible that the peptide was not properly reconstituted or contamination has occurred. In this case, do not use the solution.
 
Also, if you notice that the solution becomes cloudy or changes color over time during storage, this may be a sign of degradation or contamination, and you should not use such a solution.

It is generally not recommended to mix different peptides in the same syringe unless it is specifically stated that they are compatible. The reasons for this are:
 
Chemical incompatibility – Peptides may react with each other and lose effectiveness
Different pH requirements – Some peptides are stable under different pH conditions
Different doses – It is harder to dose precisely when peptides are mixed
Different dosing schedules – Some peptides are taken more frequently than others
 
If you are using multiple peptides, it is recommended to administer them separately, with a gap of at least 30 minutes between injections, or according to specific instructions for individual peptides.

Air bubbles in the syringe can affect dosing accuracy and may cause discomfort during injection. Here’s how to remove them:
 
1. Hold the syringe with the needle pointing upward
2. Gently tap the syringe with your finger so the bubbles rise to the top
3. Slowly push the plunger to expel air from the syringe
4. Stop when you see the first drop of liquid coming out of the needle
 
Small bubbles that remain in the syringe are usually not a problem for subcutaneous injections. However, for intramuscular injections, it is more important to remove all bubbles.

Proper disposal of needles and syringes is important for preventing injuries and the spread of infections:
 
Use a sharps container – Special containers can be purchased at pharmacies
Never recap the needle – This increases the risk of needle sticks
Do not throw needles and syringes in regular trash – This poses a risk to others
When the container is 3/4 full – Close it and dispose of it according to local regulations
 
If you do not have a sharps container, you can temporarily use a sturdy plastic bottle with a lid (e.g., a detergent bottle). Label it as “MEDICAL WASTE” and keep it out of reach of children.
 
Many pharmacies and healthcare facilities have programs for safe disposal of medical waste. Inquire about options in your area.